Difference between revisions of "Part:BBa K208051"

 
 
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<partinfo>BBa_K208051 short</partinfo>
 
<partinfo>BBa_K208051 short</partinfo>
  
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HlyA Signal Peptide with an attached Terminator
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This is a GFP composite with a TorA signal peptide, promoter, RBS, and terminator.  This is in pSB1AK3.
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NOTE: The scar between the signal peptide and the protein is not presented correctly in the sequence below. The scar is the Silver-fusion scar (ACTAGA), which allows for in-frame fusion. Additionally, the scars before the signal peptide and after the protein are affected by these parts each having the Silver-fusion prefix and suffix.
  
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===Usage and Biology===
 
===Usage and Biology===
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This part can be used to demonstrate GFP secretion via the Tat pathway.
  
 
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Latest revision as of 01:12, 23 October 2009

Tet Promoter/RBS/TorA/GFP/Terminator

This is a GFP composite with a TorA signal peptide, promoter, RBS, and terminator. This is in pSB1AK3.

NOTE: The scar between the signal peptide and the protein is not presented correctly in the sequence below. The scar is the Silver-fusion scar (ACTAGA), which allows for in-frame fusion. Additionally, the scars before the signal peptide and after the protein are affected by these parts each having the Silver-fusion prefix and suffix.

Usage and Biology

This part can be used to demonstrate GFP secretion via the Tat pathway.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 216
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 735
    Illegal XhoI site found at 636
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]