Difference between revisions of "Part:BBa K210011:Design"

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Hirohide Saito
 
Hirohide Saito
 +
 +
Possible ideal lac operator: Escherichia coli lac operator-like
 +
sequences from eukaryotic genomes lack the central GC pair
 +
(lac repressor/protein-DNA recognition/symmetry/molecular cloning)
 +
 +
ANNEMARIE SIMONS

Latest revision as of 16:25, 20 October 2009

LacI binding sites' tandem sequence #200


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This parts was made by MPR. Due to mutation, we could not suceed to sequence. We confirmed this has only one set of EcoR1, Xba1, Spe1 and Pst1 recognition sites.



Source

primer

References

Motif programming: a microgene-based method for creating synthetic proteins containing multiple functional motifs

Hirohide Saito

Possible ideal lac operator: Escherichia coli lac operator-like sequences from eukaryotic genomes lack the central GC pair (lac repressor/protein-DNA recognition/symmetry/molecular cloning)

ANNEMARIE SIMONS