Difference between revisions of "Part:BBa K208049:Design"

 
 
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__NOTOC__
 
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<partinfo>BBa_K208049 short</partinfo>
 
<partinfo>BBa_K208049 short</partinfo>
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===Design Notes===
 
===Design Notes===
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Silver Fusion
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GFP with secretion tag, promoter, RBS, and terminator.  The GFP and secretion tag are both Silver-fusion compatible. The plasmid used is pSB1AK3. Please consult the original BioBrick pages for more detailed information on their functionality.
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===Source===
 
===Source===
  
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Composite
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This is a composite part constructed from multiple BioBricks either already present in the registry or recently added by our 2009 team.
  
 
===References===
 
===References===
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1. Barrett CML, Ray N, Thomas JD, Robinson C, Bolhuis A (2003) Quantitative export of a reporter protein, GFP, by the twin-arginine translocation pathway in Escherichia coli. Biochem BIophys Res Comm 304:279-284<br>
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2. Santini C, Bernadac A, Zhang M, Chanal A, Ize B, Blan co C, Wu L (2001) Translocation of jellyfish green fluorescent protein via the Tat system of Escherichia coli and change of its periplasmic localization in response to osmotic up-shock. J Biol Chem 276:8159-8164<br>
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3. Thomas JD, Daniel RA, errington J, Robinson C (2001) Export of Active green fluorescent protein to the periplasm by the twin-arginine translocase (Tat) pathway in Escherichia coli. Mol Microbiol 39:47-53

Latest revision as of 16:08, 22 October 2009

Lac Promoter/RBS/TorA/GFP/Term


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 362
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 881
    Illegal XhoI site found at 782
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

GFP with secretion tag, promoter, RBS, and terminator. The GFP and secretion tag are both Silver-fusion compatible. The plasmid used is pSB1AK3. Please consult the original BioBrick pages for more detailed information on their functionality.

Source

This is a composite part constructed from multiple BioBricks either already present in the registry or recently added by our 2009 team.

References

1. Barrett CML, Ray N, Thomas JD, Robinson C, Bolhuis A (2003) Quantitative export of a reporter protein, GFP, by the twin-arginine translocation pathway in Escherichia coli. Biochem BIophys Res Comm 304:279-284
2. Santini C, Bernadac A, Zhang M, Chanal A, Ize B, Blan co C, Wu L (2001) Translocation of jellyfish green fluorescent protein via the Tat system of Escherichia coli and change of its periplasmic localization in response to osmotic up-shock. J Biol Chem 276:8159-8164
3. Thomas JD, Daniel RA, errington J, Robinson C (2001) Export of Active green fluorescent protein to the periplasm by the twin-arginine translocase (Tat) pathway in Escherichia coli. Mol Microbiol 39:47-53