Difference between revisions of "Part:BBa K4830032:Design"
Aruzhan001 (Talk | contribs) (→References) |
Aruzhan001 (Talk | contribs) |
||
| (One intermediate revision by the same user not shown) | |||
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | + | No alterations have been made to the original sequence. In order to use it for prime editing, this RT was fused to nickase Cas9 and combined with CMV promoter and plasmid backbone. | |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
| − | + | NCBI ACCESSION: NP_056728 | |
===References=== | ===References=== | ||
Franck, A. et al. (1980) ‘Nucleotide sequence of cauliflower mosaic virus DNA’, Cell, 21(1), pp. 285–294. doi:10.1016/0092-8674(80)90136-1. | Franck, A. et al. (1980) ‘Nucleotide sequence of cauliflower mosaic virus DNA’, Cell, 21(1), pp. 285–294. doi:10.1016/0092-8674(80)90136-1. | ||
Latest revision as of 03:22, 12 October 2023
CaMV RT
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
No alterations have been made to the original sequence. In order to use it for prime editing, this RT was fused to nickase Cas9 and combined with CMV promoter and plasmid backbone.
Source
NCBI ACCESSION: NP_056728
References
Franck, A. et al. (1980) ‘Nucleotide sequence of cauliflower mosaic virus DNA’, Cell, 21(1), pp. 285–294. doi:10.1016/0092-8674(80)90136-1.