Difference between revisions of "Part:BBa K283011"
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<partinfo>BBa_K283011 short</partinfo> | <partinfo>BBa_K283011 short</partinfo> | ||
| − | The fusion protein lpp-ompA(46-159)-streptavidin(WT) with a 6-histidine tail is linked after a pTet promoter. The fusion protein coding region does not contain any terminator. The backbone of this biobrick is based on BBa_J61003.lpp_ompA(46-159)_streptavidin(WT)_his tag lies between XbaI and SpeI. | + | The fusion protein lpp-ompA(46-159)-streptavidin(WT) with a 6-histidine tail is linked after a pTet promoter. The fusion protein coding region does not contain any terminator. The backbone of this biobrick is based on BBa_J61003.lpp_ompA(46-159)_streptavidin(WT)_his tag lies between XbaI and SpeI.This part does not pose any biological threat. |
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 11:59, 21 October 2009
pTet-rbs-lpp_omp(46-159)_streptavidin_Histag
The fusion protein lpp-ompA(46-159)-streptavidin(WT) with a 6-histidine tail is linked after a pTet promoter. The fusion protein coding region does not contain any terminator. The backbone of this biobrick is based on BBa_J61003.lpp_ompA(46-159)_streptavidin(WT)_his tag lies between XbaI and SpeI.This part does not pose any biological threat.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 565
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 607
Illegal AgeI site found at 658 - 1000COMPATIBLE WITH RFC[1000]