Difference between revisions of "Part:BBa K283003"

Latest revision as of 11:58, 21 October 2009

pTet-rbs-chez_histag-double terminator

This part is an improved version of BBa_K094102. BBa_K094102 was designed to express CheZ protein, which is responsible for the dephosphorylation of CheY, a major effector in bacterial flagella rotation. cheZ gene is driven by the pTet promoter, whose expression is induced by adding anhydrotetracycline (aTc). The backbone of this biobrick is BBa_J61003. A strong double terminators lie between XbaI and SpeI sites.

Since lacking an antibody to recognize the expressed CheZ, we fused a His6-tag to the cheZ encoding region. Thus, the expression of cheZ can be easily detected by immunostainning and western blotting analysis. And this part has been demonstrated work as expected in our system (you can get more information in our wiki).This part does not pose any biological threat.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 726
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K283003 short</partinfo>
-This part was designed to generate the cheZ proteins which are responsible for the dephosphorylation of cheY protein involved in bacteria flagella movement. A 6-histidine tail is added to the cheZ encoding region. The pTet can be repressed by the TetR repressor protein(C0040). It is constitutively on without the presence of TetR repressor. The backbone of this biobrick is based on BBa_J61003.  CheZ_his tag and strong double terminators lie between XbaI and SpeI.
+This part is an improved version of BBa_K094102. BBa_K094102 was designed to express CheZ protein, which is responsible for the dephosphorylation of CheY, a major effector in bacterial flagella rotation. '' cheZ'' gene is driven by the pTet promoter, whose expression is induced by adding anhydrotetracycline (aTc). The backbone of this biobrick is BBa_J61003. A strong double terminators lie between ''Xba''I and ''Spe''I sites.
+Since lacking an antibody to recognize the expressed CheZ, we fused a His6-tag to the ''cheZ'' encoding region. Thus, the expression of ''cheZ'' can be easily detected by immunostainning and western blotting analysis. And this part has been demonstrated work as expected in our system (you can get more information in our wiki).This part does not pose any biological threat.