Difference between revisions of "Part:BBa K4837001:Design"
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The biobricks contained in our plasmid are illustrated in the above graph. Two basic parts we designed, including BBa_K4837000 and BBa_K4837001 are used to synthesize lignin peroxidase (LiP) and manganese peroxidase (MnP) respectively.
  
 
pSBIK3 vector was adopted in this plasmid.The promoter used in these two basic parts was arabinose promoter. Purification tag and fluorescent tag were also included for better detection of the optimized enzyme synthesized.
 
pSBIK3 vector was adopted in this plasmid.The promoter used in these two basic parts was arabinose promoter. Purification tag and fluorescent tag were also included for better detection of the optimized enzyme synthesized.
  
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Other biobricks such as BBa_K4837000 are required to perform its function.
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Other biobricks such as BBa_K4837000 are required to perform its function. The details of these two basic parts are listed below:
  
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<a href='https://static.igem.wiki/teams/4837/wiki/part-table.jpg' target="_blank"><img src="https://static.igem.wiki/teams/4837/wiki/part-table.jpg" width="100%"/>
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===Source===
 
===Source===

Latest revision as of 10:50, 12 October 2023


Manganese peroxidase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 126
    Illegal NotI site found at 781
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 66
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1149
    Illegal NgoMIV site found at 1153
    Illegal AgeI site found at 651
    Illegal AgeI site found at 1132
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 865
    Illegal SapI.rc site found at 523


Design Notes

The biobricks contained in our plasmid are illustrated in the above graph. Two basic parts we designed, including BBa_K4837000 and BBa_K4837001 are used to synthesize lignin peroxidase (LiP) and manganese peroxidase (MnP) respectively.

pSBIK3 vector was adopted in this plasmid.The promoter used in these two basic parts was arabinose promoter. Purification tag and fluorescent tag were also included for better detection of the optimized enzyme synthesized.

Other biobricks such as BBa_K4837000 are required to perform its function. The details of these two basic parts are listed below:

Source

It is designed based on part of white-rot fungus (Phanerochaete chrysosporium).

References