Difference between revisions of "Part:BBa K283000"
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| − | This | + | Streptavidin protein binds strongly to the biotin molecule, and is widely used to bind biotinylated materials. This device was designed for polar expression of streptavidin on the outer membrane of bacterial cells. To facilitate the observation, the fusion protein of Lpp signal peptide, OmpA aa46-66, and streptavidin wild-type (BBa_J36848) was fused with GFP-coding sequence (BBa_e0040). The expression will display streptavidin-GFP fusion protein on the cell surface. It has been demonstrated work as expected in our system (see experience or visit our wiki). This part does not pose any biological threat. |
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Latest revision as of 12:10, 21 October 2009
pTet-rbs-lpp_ompA_eGFP_streptavidin-double Terminators
Streptavidin protein binds strongly to the biotin molecule, and is widely used to bind biotinylated materials. This device was designed for polar expression of streptavidin on the outer membrane of bacterial cells. To facilitate the observation, the fusion protein of Lpp signal peptide, OmpA aa46-66, and streptavidin wild-type (BBa_J36848) was fused with GFP-coding sequence (BBa_e0040). The expression will display streptavidin-GFP fusion protein on the cell surface. It has been demonstrated work as expected in our system (see experience or visit our wiki). This part does not pose any biological threat.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1272
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1314
Illegal AgeI site found at 1365 - 1000COMPATIBLE WITH RFC[1000]