Difference between revisions of "Part:BBa K4825046"
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<partinfo>BBa_K4825046 short</partinfo> | <partinfo>BBa_K4825046 short</partinfo> | ||
− | This composite part contains the part ARO10 BBa_K4825015, ADH7 BBa_K4825016 and ATF1 BBa_K4825017. In the pathway of producing isoamyl acetate in S. cerevisiae, ARO10 encodes for 2-oxoacid decarboxylase, which is reponsible for converting 2-ketoisocaproate (KIC) to isopentaldehyde. ADH7, which encodes for alcohol dehydrogenase 7, is responsible for converting isopentaldehyde to isopantanol. ATF1 encodes for alcohol acetyltransferase, which catalyzes the conversion of isopenanol to isopentyl acetate (isoamyl acetate). | + | This composite part contains the part ARO10 BBa_K4825015, ADH7 BBa_K4825016 and ATF1 BBa_K4825017. In the pathway of producing isoamyl acetate in <i>S. cerevisiae</i>, ARO10 encodes for 2-oxoacid decarboxylase, which is reponsible for converting 2-ketoisocaproate (KIC) to isopentaldehyde. ADH7, which encodes for alcohol dehydrogenase 7, is responsible for converting isopentaldehyde to isopantanol. ATF1 encodes for alcohol acetyltransferase, which catalyzes the conversion of isopenanol to isopentyl acetate (isoamyl acetate). |
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Latest revision as of 12:18, 12 October 2023
ARO10-ADH7-ATF1
This composite part contains the part ARO10 BBa_K4825015, ADH7 BBa_K4825016 and ATF1 BBa_K4825017. In the pathway of producing isoamyl acetate in S. cerevisiae, ARO10 encodes for 2-oxoacid decarboxylase, which is reponsible for converting 2-ketoisocaproate (KIC) to isopentaldehyde. ADH7, which encodes for alcohol dehydrogenase 7, is responsible for converting isopentaldehyde to isopantanol. ATF1 encodes for alcohol acetyltransferase, which catalyzes the conversion of isopenanol to isopentyl acetate (isoamyl acetate).
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1791
Illegal EcoRI site found at 3418
Illegal SpeI site found at 742
Illegal PstI site found at 620 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1791
Illegal EcoRI site found at 3418
Illegal NheI site found at 2385
Illegal SpeI site found at 742
Illegal PstI site found at 620 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1791
Illegal EcoRI site found at 3418
Illegal BglII site found at 3105
Illegal BamHI site found at 2146
Illegal BamHI site found at 4432 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1791
Illegal EcoRI site found at 3418
Illegal SpeI site found at 742
Illegal PstI site found at 620 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1791
Illegal EcoRI site found at 3418
Illegal SpeI site found at 742
Illegal PstI site found at 620 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 3532
Illegal SapI.rc site found at 4540