Difference between revisions of "Part:BBa K4830017:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | No alterations have been made to the original sequence. In order to use it for prime editing, this RT was fused to nickase Cas9 and combined with CMV promoter and plasmid backbone. | |
===Source=== | ===Source=== | ||
Line 14: | Line 14: | ||
===References=== | ===References=== | ||
+ | López-González, M.J. et al. (2018) ‘Adaptive evolution of Industrial Lactococcus lactis under cell envelope stress provides phenotypic diversity’, Frontiers in Microbiology, 9. doi:10.3389/fmicb.2018.02654. |
Latest revision as of 01:42, 12 October 2023
Ll.ltrA RT
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
No alterations have been made to the original sequence. In order to use it for prime editing, this RT was fused to nickase Cas9 and combined with CMV promoter and plasmid backbone.
Source
NCBI ACCESSION: RQG78521
References
López-González, M.J. et al. (2018) ‘Adaptive evolution of Industrial Lactococcus lactis under cell envelope stress provides phenotypic diversity’, Frontiers in Microbiology, 9. doi:10.3389/fmicb.2018.02654.