Difference between revisions of "Part:BBa K4788002:Design"
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===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
| + | Ma Y, Zheng X, Lin Y, Zhang L, Yuan Y, Wang H, Winterburn J, Wu F, Wu Q, Ye JW, Chen GQ. Engineering an oleic acid-induced system for Halomonas, E. coli and Pseudomonas. Metab Eng. 2022 Jul;72:325-336. doi: 10.1016/j.ymben.2022.04.003. Epub 2022 May 2. PMID: 35513297. | ||
Latest revision as of 21:54, 11 October 2023
Oleic acid inducer
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 764
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The front of this part requires a promoter to express the FadR protein in order to achieve oleic acid response function. Therefore, in practical use, appropriate promoters or plasmid skeletons should be selected. In longer-term applications, we can try to change the number and position of FadO operators to change the oleic acid induction threshold to achieve more precise promoter regulation.
Source
We synthesized it
References
Ma Y, Zheng X, Lin Y, Zhang L, Yuan Y, Wang H, Winterburn J, Wu F, Wu Q, Ye JW, Chen GQ. Engineering an oleic acid-induced system for Halomonas, E. coli and Pseudomonas. Metab Eng. 2022 Jul;72:325-336. doi: 10.1016/j.ymben.2022.04.003. Epub 2022 May 2. PMID: 35513297.