Difference between revisions of "Part:BBa F1610"

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'''We use this part on iGEM2009 plate. While present on a gel after cutting the miniprep DNA extraction by EcoRI and SpeI, the restriction fragment appears to about 3000 bp and 800 bp . BBa_F1610 on iGEM2009 plate is therefore correct.'''--[[User:Koi|Koi]]  20 Oct 2009 (Tokyo_Tech)
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'''The part we used is in iGEM2009 plate. After cutting the plasmid in EcoRI and Spel restriction sites, we separated the fragments by electrophoresis. There appeared two bands, one is about 3100bp, and the other is about 800bp.'''--[[User:Koi|Koi]]  20 Oct 2009 (Tokyo_Tech)
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[[:Image:F1610_restriction2009.jpg|See gel|image]]
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[[:Image:F1610_restriction2009.jpg|See gel image]]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 10:09, 20 October 2009

3OC6HSL Sender Device

This device accepts PoPS as input and produces the LuxI enzyme. This enzyme produces 3OC6HSL.

Notice

With a VF2-VR PCR length compraison, I found that it is empty on this plasmid sent in iGEM2007 plates. --marion 07:12, 22 March 2008 (EDT)


While present on a gel after running PCR on both 2007 and 2008 plates, the DNA appears to only be ~600bp long which conflicts with the part description. --robere 27 Jun 2008
See gel image


The part we used is in iGEM2009 plate. After cutting the plasmid in EcoRI and Spel restriction sites, we separated the fragments by electrophoresis. There appeared two bands, one is about 3100bp, and the other is about 800bp.--Koi 20 Oct 2009 (Tokyo_Tech)

See gel image

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 655
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]