Difference between revisions of "Part:BBa K4676000:Design"

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===Design Notes===
 
===Design Notes===
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There are different versions of the RM1 and RM2 motiffs in the h-fibroin gene. When creating this part, only the first RM1 version that shows up in the gene was included, and only the first RM2 version that shows up in the gene was included. Furthermore, this sequence has been codon optimized using the biotechnology platform Benchling.
 
There are different versions of the RM1 and RM2 motiffs in the h-fibroin gene. When creating this part, only the first RM1 version that shows up in the gene was included, and only the first RM2 version that shows up in the gene was included. Furthermore, this sequence has been codon optimized using the biotechnology platform Benchling.
  
 
===Source===
 
===Source===
  
−
This sequence was created by truncating the much larger h-fibroin sequence, which was published as supplementary information by Heckenhauer et al. for their paper "Characterization of the primary structure of the major silk gene, h-fibroin, across caddisfly (Trichoptera) suborders."
+
This sequence was created by truncating the much larger h-fibroin sequence, which was published as supplementary information by Heckenhauer et al. for their paper "Characterization of the primary structure of the major silk gene, h-fibroin, across caddisfly (Trichoptera) suborders." (1)
  
 
===References===
 
===References===
 +
1. Heckenhauer, J., & Stewart, R. J., et al. (2023, August 18). Characterization of the primary structure of the major silk gene, h-fibroin, across caddisfly (Trichoptera) suborders. iScience. Cell Press. https://www.cell.com/iscience/pdf/S2589-0042(23)01330-5.pdf

Latest revision as of 00:46, 12 October 2023


Truncated Control H-Fibroin of G. Pellucidus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There are different versions of the RM1 and RM2 motiffs in the h-fibroin gene. When creating this part, only the first RM1 version that shows up in the gene was included, and only the first RM2 version that shows up in the gene was included. Furthermore, this sequence has been codon optimized using the biotechnology platform Benchling.

Source

This sequence was created by truncating the much larger h-fibroin sequence, which was published as supplementary information by Heckenhauer et al. for their paper "Characterization of the primary structure of the major silk gene, h-fibroin, across caddisfly (Trichoptera) suborders." (1)

References

1. Heckenhauer, J., & Stewart, R. J., et al. (2023, August 18). Characterization of the primary structure of the major silk gene, h-fibroin, across caddisfly (Trichoptera) suborders. iScience. Cell Press. https://www.cell.com/iscience/pdf/S2589-0042(23)01330-5.pdf