Difference between revisions of "Part:BBa K4601007:Design"
Line 10: | Line 10: | ||
===Source=== | ===Source=== | ||
− | DNA synthesis + PCR amplifications from ''N. pharaonis'' and ''E. coli'' | + | DNA synthesis + PCR amplifications from ''N. pharaonis'' and ''E. coli'' genomes, followed by Golden Gate assembly |
===References=== | ===References=== |
Latest revision as of 19:40, 11 October 2023
Jaws-NpHtrII-EnvZ
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 581
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 816
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 957
Design Notes
Source
DNA synthesis + PCR amplifications from N. pharaonis and E. coli genomes, followed by Golden Gate assembly