Difference between revisions of "Part:BBa K176013"

Latest revision as of 10:25, 21 October 2009

pCon 0.36(K176009)->RBS+GFP+T

pCon 0.36(K176009)+I13504

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 705

MEASUREMENT

We measured five constitutive promoter family member ( J23100, J23101, J23103, J23107, J23109, J23115, )strength by ligated each with a 
GFP gene ( I13504) , and then moniter the OD600 and GFP expression.
K176011: J23100 + GFP ( I13504 )
K176012: J23101 + GFP ( I13504)
K176013: K176009( J23107) + GFP ( I13504)
K176014: K176008( J23115) + GFP ( I13504)
K176015: J23109 + GFP ( I13504)
K176016: J23103 + GFP ( I13504)

Model

To see the details of the model, click [http://2009.igem.org/Team:USTC/Modeling/Model-1 here] please.

data

J23107+GFP

COLONY NUMBER	T(min)	OD600	FLU(arbitrary)	ST.FLU(ustc_st.1)	FLU/OD600(arbitrary)	ST.FLU/OD600(ustc_st.1)
Ⅰ	0	0.335	75.804	303.0099532	226.280597	904.5073231
Ⅰ	30	0.405	95.60666667	382.1667932	236.0658436	943.6217117
Ⅰ	60	0.485	119	475.67654	245.3608247	980.776371
Ⅰ	90	0.568	143.9033333	575.2221822	253.350939	1012.71511
Ⅰ	120	0.718	199.35	796.8581365	277.6462396	1109.830274
Ⅰ	180	0.984	266.53	1065.395531	270.8638211	1082.719036
Ⅱ	0	0.329	75.393	301.3670704	229.1580547	916.0093325
Ⅱ	30	0.404	101.95	407.5228844	252.3514851	1008.720011
Ⅱ	60	0.483	121.59	486.0294999	251.7391304	1006.272257
Ⅱ	90	0.565	147.0266667	587.7070259	260.2241888	1040.189426
Ⅱ	120	0.711	207.9833333	831.3680031	292.5222691	1169.293957
Ⅱ	180	0.97	273.8633333	1094.708931	282.3333333	1128.565909

For the details of the definition of ST.FLU and the unit ustc_st1, please click here.

plot

protocol

1. Streak a plate of the strain which contain one of the parts listed in pSB1A3 .

2. Inoculate two 3ml cultures of supplemented M9 Medium and antibiotic( Ampicillin 0.1mg/ml) with single colony from the plate.

3. Cultures were grown in test tubes（BIO BASIC INC.12ml Polypropylene Round-bottom Culture Tubes With Graduations And Dual Cap Cat.No:TD444） for 16hrs at 37℃ with shaking at 200rpm.

4. Cultures were diluted 1:100 into 3ml fresh medium and grown for 3hrs.

5. Measure the fluorescence(SHIMDZU SPECTROFLUOROPHOTOMETER RF-5301PC, 250ul quartz cell path length 10mm,501 nm excitation,514 nm emission,1.5nm slit width) and absorbance (HITACHI UV-VIS spectrophotometer U-2810 ,200ul quartz cell,path length 10mm,600nm,1.5 nm slit width) every 30 minutes in the next 4hrs.

@@ Line 20: / Line 20: @@
   K176015: J23109 + GFP ( I13504)
   K176016: J23103 + GFP ( I13504)
+==Model==
+To see the details of the model, click [http://2009.igem.org/Team:USTC/Modeling/Model-1 here] please.
 ===data===
 J23107+GFP