Difference between revisions of "Part:BBa K4825024"
 
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pXyl is a synthetic inducible promoter that is regulated by inducer xylose. It is constructed to be the minimal inducible promoter in eukaryotes.  
 
pXyl is a synthetic inducible promoter that is regulated by inducer xylose. It is constructed to be the minimal inducible promoter in eukaryotes.  
  
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This part is sensitive to xylose and repressed by glucose, but insensitive to other metabolites. It is associated with the xylose-dependent activator XylR. The galactose inductibility of this part has been widely used in heterologous protein expression. In our project, this part is utilized to construct a plasmid with Bax gene to fulfill the kill switch design with a logic gate induced by xylose in S.cerevisiae.  
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This part is sensitive to xylose and repressed by glucose, but insensitive to other metabolites. It is associated with the xylose-dependent activator XylR. The galactose inductibility of this part has been widely used in heterologous protein expression. In our project, this part is utilized to construct a plasmid with Bax gene to fulfill the kill switch design with a logic gate induced by xylose in S.cerevisiae.  
  
  

Latest revision as of 18:54, 11 October 2023


pXyl

pXyl is a synthetic inducible promoter that is regulated by inducer xylose. It is constructed to be the minimal inducible promoter in eukaryotes.

This part is sensitive to xylose and repressed by glucose, but insensitive to other metabolites. It is associated with the xylose-dependent activator XylR. The galactose inductibility of this part has been widely used in heterologous protein expression. In our project, this part is utilized to construct a plasmid with Bax gene to fulfill the kill switch design with a logic gate induced by xylose in S.cerevisiae.


Usage and Biology

pXyl is a hybrid promoter with two operators XylO1 that is constructed in S.cerevisiae. Its regulatory protein, XylR, modified from Escherichia coli, binds to XylO1 when xylose is present in the cellular environment, further enhancing the transcriptional level of the xylose promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1
  • 1000
    COMPATIBLE WITH RFC[1000]