Difference between revisions of "Part:BBa K176011"

Latest revision as of 10:22, 21 October 2009

pCon 1.00(J23100)->RBS+GFP+T

pCon 1.00(J23100)+I13504

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 705

MEASUREMENT

We measured five constitutive promoter family member ( J23100, J23101, J23103, J23107, J23109, J23115, )strength by ligated each with a 
GFP gene ( I13504) , and then moniter the OD600 and GFP expression.
K176011: J23100 + GFP ( I13504 )
K176012: J23101 + GFP ( I13504)
K176013: K176009( J23107) + GFP ( I13504)
K176014: K176008( J23115) + GFP ( I13504)
K176015: J23109 + GFP ( I13504)
K176016: J23103 + GFP ( I13504)

model

To see the details of the model, please click [http://2009.igem.org/Team:USTC/Modeling/Model-1 here]

data

J23100+GFP

COLONY NUMBER	T(min)	OD600	FLU(arbitrary)	ST.FLU(ustc_st.1)	FLU/OD600(arbitrary)	ST.FLU/OD600(ustc_st.1)
Ⅰ	0	0.071	132.07	527.9210137	1860.140845	7435.507235
Ⅰ	30	0.076	144.49	577.5672543	1901.184211	7599.569135
Ⅰ	60	0.101	157.6633333	630.2247805	1561.023102	6239.849312
Ⅰ	90	0.093	160.8866667	643.1093523	1729.964158	6915.154326
Ⅰ	120	0.116	176.33	704.8407083	1520.086207	6076.213003
Ⅰ	150	0.122666667	185.0633333	739.7503031	1508.668478	6030.573123
Ⅰ	180	0.153	197.8966667	791.0487535	1293.442266	5170.253291
Ⅱ	0	0.07	144.1	576.0083143	2058.571429	8228.690205
Ⅱ	30	0.075333333	136.1066667	544.0567081	1806.725664	7221.991701
Ⅱ	60	0.084333333	144.92	579.2860855	1718.418972	6869.004966
Ⅱ	90	0.092	157.4133333	629.22546	1711.014493	6839.407174
Ⅱ	120	0.098	164.73	658.4722389	1680.918367	6719.104478
Ⅱ	150	0.117	179.78	718.6313307	1536.581197	6142.148126
Ⅱ	180	0.135666667	194.6566667	778.0975603	1434.815725	5735.362853

For the details of the definition of ST.FLU and the unit ustc_st1, please click here.

plot

Protocol

1. Streak a plate of the strain which contain one of the parts listed in pSB1A3 .

2. Inoculate two 3ml cultures of supplemented M9 Medium and antibiotic( Ampicillin 0.1mg/ml) with single colony from the plate.

3. Cultures were grown in test tubes（BIO BASIC INC.12ml Polypropylene Round-bottom Culture Tubes With Graduations And Dual Cap Cat.No:TD444） for 16hrs at 37℃ with shaking at 200rpm.

4. Cultures were diluted 1:100 into 3ml fresh medium and grown for 3hrs.

5. Measure the fluorescence(SHIMDZU SPECTROFLUOROPHOTOMETER RF-5301PC, 250ul quartz cell path length 10mm,501 nm excitation,514 nm emission,1.5nm slit width) and absorbance (HITACHI UV-VIS spectrophotometer U-2810 ,200ul quartz cell,path length 10mm,600nm,1.5 nm slit width) every 30 minutes in the next 4hrs.

@@ Line 12: / Line 12: @@
 <partinfo>BBa_K176011 SequenceAndFeatures</partinfo>
 =='''MEASUREMENT'''==
-===DATA===
+ We measured five '''constitutive promoter family member''' ( J23100, J23101, J23103, J23107, J23109, J23115, )strength by ligated each with a
+ GFP gene ( I13504) , and then moniter the OD600 and GFP expression.
+ K176011: J23100 + GFP ( I13504 )
+ K176012: J23101 + GFP ( I13504)
+ K176013: K176009( J23107) + GFP ( I13504)
+ K176014: K176008( J23115) + GFP ( I13504)
+ K176015: J23109 + GFP ( I13504)
+ K176016: J23103 + GFP ( I13504)
+===model===
+To see the details of the model, please click [http://2009.igem.org/Team:USTC/Modeling/Model-1 here]
+===data===
+J23100+GFP
 {| {{table}}
-{|class="sortable" style="border:3px solid black; width: 70%"
+{|class="sortable" style="border:3px solid black; width: 70%" align="center"
 | align="center" style="background:#f0f0f0;"|'''COLONY NUMBER'''
 | align="center" style="background:#f0f0f0;"|'''T(min)'''
@@ Line 54: / Line 68: @@
   For the details of the definition of '''ST.FLU''' and the unit '''ustc_st1''', please click [[here]].
-===PLOT===
+===plot===
-{{USTC/promoter+GFP}}
+{{USTC//promoter+GFP}}
+===Protocol===
+{{:Team:USTC/Template/promoter protocols}}