Difference between revisions of "Part:BBa K177002"
 
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<partinfo>BBa_K177002 short</partinfo>
 
<partinfo>BBa_K177002 short</partinfo>
  
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Cro-box is the particular fragment of phage lambda DNA which is recognized by cro repressor protein. This sequence is an artificially designed (based on natural fragment of lambda phage genome)
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Cro-box is the particular fragment of phage lambda DNA which is recognized by cro repressor protein. This sequence is artificially designed (based on natural fragment of lambda phage genome) to increase the affinity for cro protein.
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===Usage and Biology===
 
===Usage and Biology===
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When this part is introduced into a sequence of elements expressed from a single promoter the binding of the Cro protein (<partinfo>K177009</partinfo>) successfully inhibits the expression of elements located downstream.
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An alternative version of this sequence has also been created (<partinfo>K177047</partinfo>) which differs from this one by the binding strength of repressor proteins, Cro (<partinfo>K177009</partinfo>) and cI (<partinfo>C0051</partinfo>; <partinfo>K177050</partinfo>).
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K177002 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K177002 SequenceAndFeatures</partinfo>

Latest revision as of 15:03, 21 October 2009

cro binding box

Cro-box is the particular fragment of phage lambda DNA which is recognized by cro repressor protein. This sequence is artificially designed (based on natural fragment of lambda phage genome) to increase the affinity for cro protein.


Usage and Biology

When this part is introduced into a sequence of elements expressed from a single promoter the binding of the Cro protein (BBa_K177009) successfully inhibits the expression of elements located downstream.

An alternative version of this sequence has also been created (BBa_K177047) which differs from this one by the binding strength of repressor proteins, Cro (BBa_K177009) and cI (BBa_C0051; BBa_K177050).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]