Difference between revisions of "Part:BBa K4825026:Design"

 
 
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===Source===
 
===Source===
  
−
Bacillus licheniformis
+
<i>Bacillus licheniformis</i>
  
 
===References===
 
===References===
 +
"Chen, Y., Zhang, S., Young, E.M. et al. Genetic circuit design automation for yeast. Nat Microbiol 5, 1349–1360 (2020). https://doi.org/10.1038/s41564-020-0757-2.
 +
 +
Wei W, Shang Y, Zhang P, Liu Y, You D, Yin B, Ye B. Engineering Prokaryotic Transcriptional Activator XylR as a Xylose-Inducible Biosensor for Transcription Activation in Yeast. ACS Synth Biol. 2020 May 15;9(5):1022-1029. doi: 10.1021/acssynbio.0c00122. Epub 2020 Apr 17. PMID: 32268060."

Latest revision as of 12:10, 12 October 2023


XylR-NLS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 235
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

LEU2 is used as the yeast auxotrophic marker in this part, instead of normally URA3, which allows the co-transformation of the plasmid of this part and the plasmid of xylose promoters.


Source

Bacillus licheniformis

References

"Chen, Y., Zhang, S., Young, E.M. et al. Genetic circuit design automation for yeast. Nat Microbiol 5, 1349–1360 (2020). https://doi.org/10.1038/s41564-020-0757-2.

Wei W, Shang Y, Zhang P, Liu Y, You D, Yin B, Ye B. Engineering Prokaryotic Transcriptional Activator XylR as a Xylose-Inducible Biosensor for Transcription Activation in Yeast. ACS Synth Biol. 2020 May 15;9(5):1022-1029. doi: 10.1021/acssynbio.0c00122. Epub 2020 Apr 17. PMID: 32268060."