Difference between revisions of "Part:BBa K4960011"
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__NOTOC__ | __NOTOC__ | ||
| − | <partinfo>BBa_K4960011 short</partinfo> | + | <partinfo>BBa_K4960011 short</partinfo><br> |
| + | Pvc11 is a part of pvc baseplate. | ||
===Profile=== | ===Profile=== | ||
Name: pvc11<br> | Name: pvc11<br> | ||
Base Pairs: 2187bp<br> | Base Pairs: 2187bp<br> | ||
| − | Origin: Photorhabdus<br> | + | Origin: <i>Photorhabdus</i><br> |
Properties: Pvc11 is a part of pvc baseplate.<br> | Properties: Pvc11 is a part of pvc baseplate.<br> | ||
<!-- Add more about the biology of this part here--> | <!-- Add more about the biology of this part here--> | ||
| + | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices for targeted delivery of proteins .[1]Structurally similar to other CISs, PVCs are also macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2](Figure1)<br> | + | Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices for targeted delivery of proteins .[1]Structurally similar to other CISs, PVCs are also macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2]('''Figure1''')<br> |
<html> | <html> | ||
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</html> | </html> | ||
| − | Figure 1. Schematic of the PVCpnf locus. It contains 16 structural and accessory genes , followed by two payload genes (Pdp1 and Pnf, in red) and four putative regulatory genes (in pink).<br> | + | '''Figure 1. Schematic of the PVCpnf locus.''' It contains 16 structural and accessory genes , followed by two payload genes (Pdp1 and Pnf, in red) and four putative regulatory genes (in pink).<br> |
<br> | <br> | ||
In the assembled wedges, six copies of Pvc11-Pvc12 heterodimers form a hexagonal ring through the dimerization between the domain V of pvc11 and the domain III of pvc12 from a neighboring heterodimer.<br> | In the assembled wedges, six copies of Pvc11-Pvc12 heterodimers form a hexagonal ring through the dimerization between the domain V of pvc11 and the domain III of pvc12 from a neighboring heterodimer.<br> | ||
Latest revision as of 14:06, 12 October 2023
pvc11
Pvc11 is a part of pvc baseplate.
Profile
Name: pvc11
Base Pairs: 2187bp
Origin: Photorhabdus
Properties: Pvc11 is a part of pvc baseplate.
Usage and Biology
Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices for targeted delivery of proteins .[1]Structurally similar to other CISs, PVCs are also macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2](Figure1)
In the assembled wedges, six copies of Pvc11-Pvc12 heterodimers form a hexagonal ring through the dimerization between the domain V of pvc11 and the domain III of pvc12 from a neighboring heterodimer.
Using an extrusion for the interactions with adjacent domains with pvc11.
One part involved in the association between the wedges and the central hub.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 891
Illegal AgeI site found at 1577
Illegal AgeI site found at 1718 - 1000COMPATIBLE WITH RFC[1000]
References
[1]Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Programmable protein delivery with a bacterial contractile injection system. Nature. 2023 Apr;616(7956):357-364. doi: 10.1038/s41586-023-05870-7. Epub 2023 Mar 29.
[2]Jiang F, Li N, Wang X, Cheng J, Huang Y, Yang Y, Yang J, Cai B, Wang YP, Jin Q, Gao N. Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System. Cell. 2019 Apr 4;177(2):370-383.e15. doi: 10.1016/j.cell.2019.02.020. Epub 2019 Mar 21.