Difference between revisions of "Part:BBa K4825003:Design"

 
 
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===Source===
 
===Source===
  
−
Pinus taeda
+
<i>Pinus taeda</i>
  
 
===References===
 
===References===
 +
CHEN Tianhua, ZHANG Ruosi, JIANG Guozhen, YAO Mingdong, LIU Hong, WANG Ying, XIAO Wenhai, YUAN Yingjin. Metabolic engineering of Saccharomyces cerevisiae for pinene production[J]. CIESC Journal, 2019, 70(1): 179-188

Latest revision as of 11:48, 12 October 2023


PS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 223
    Illegal BamHI site found at 1060
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 695


Design Notes

"1. The 51-position prolinate site at the N terminal of PtPS was truncated to increase the efficiency of pinene production. 2. PS is linked to ERG20ww with a flexible linker (GGGGSGGGGS) to increase the production of pinene from GPP, and reduce the GPP allocated to produce FPP to increase the yield of target product."


Source

Pinus taeda

References

CHEN Tianhua, ZHANG Ruosi, JIANG Guozhen, YAO Mingdong, LIU Hong, WANG Ying, XIAO Wenhai, YUAN Yingjin. Metabolic engineering of Saccharomyces cerevisiae for pinene production[J]. CIESC Journal, 2019, 70(1): 179-188