Difference between revisions of "Part:BBa K283023:Design"

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__NOTOC__
 
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<partinfo>BBa_K283023 short</partinfo>
 
<partinfo>BBa_K283023 short</partinfo>
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The gene expression cheZ is controled by the cI regulated promoter K094002 which is based on the pR promoter from bacteriophage lambda. The promoter has two DNA binding sites for lambda cI repressor. cI binding results in repression of transcription. Our group intended to use this device to realize speed control system.
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<partinfo>BBa_K283023 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K283023 SequenceAndFeatures</partinfo>
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===Design Notes===
 
===Design Notes===
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n/a
 
  
 
===Source===
 
===Source===
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This part is composited from BBa_R0062, BBa_B0034, BBa_K094011, BBa_K094013, BBa_K094002, BBa_B0034, BBa_K094100 and BBa_K094013
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composited from R0062, B0034, K094011, K094013, K094002 and K094100
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The origin of the cheZ gene comes from E. Coli strain MG1655 chromosome.
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===References===
 
===References===

Latest revision as of 14:46, 21 October 2009

plux-rbs-CI-terminator-plambda(R-O12)-rbs-cheZ-terminator

The gene expression cheZ is controled by the cI regulated promoter K094002 which is based on the pR promoter from bacteriophage lambda. The promoter has two DNA binding sites for lambda cI repressor. cI binding results in repression of transcription. Our group intended to use this device to realize speed control system.



Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 307
    Illegal EcoRI site found at 1792
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 307
    Illegal EcoRI site found at 1792
    Illegal NheI site found at 2472
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 307
    Illegal EcoRI site found at 1792
    Illegal BglII site found at 332
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 307
    Illegal EcoRI site found at 1792
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 307
    Illegal EcoRI site found at 1792
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

This part is composited from BBa_R0062, BBa_B0034, BBa_K094011, BBa_K094013, BBa_K094002, BBa_B0034, BBa_K094100 and BBa_K094013

References