Difference between revisions of "Part:BBa K4767018"
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We created the <i>ptpA</i> knockout mutant and complementation strain to verify its function in the formation and conductivity of <i>S. oneidensis</i> MR-1 biofilm. Our results show that the ability of the mutant strain to form biofilm on both conductive (well plate) and non-conductive (anode) surfaces significantly reduced, whereas the complementation strain restored this capability. In microbial fuel cells(MFC), maximum voltage of the mutant strain raised about 36% compared to the wild type with the lower internal resistance In summary, we come to the conclusion that <i>ptpA</i> gene in <i>S. oneidensis</i> MR-1 may possibly effect the biofilm formation and it’s conductivity by meditating the polysaccharide biosynthesis. | We created the <i>ptpA</i> knockout mutant and complementation strain to verify its function in the formation and conductivity of <i>S. oneidensis</i> MR-1 biofilm. Our results show that the ability of the mutant strain to form biofilm on both conductive (well plate) and non-conductive (anode) surfaces significantly reduced, whereas the complementation strain restored this capability. In microbial fuel cells(MFC), maximum voltage of the mutant strain raised about 36% compared to the wild type with the lower internal resistance In summary, we come to the conclusion that <i>ptpA</i> gene in <i>S. oneidensis</i> MR-1 may possibly effect the biofilm formation and it’s conductivity by meditating the polysaccharide biosynthesis. | ||
| − | <center> https://static.igem.wiki/teams/4767/wiki/ | + | <center> https://static.igem.wiki/teams/4767/wiki/part/ptpa003.png </center> |
The growth curves of the wild-type(WT), ∆<i>ptpA</i> and the complementaion strain(Fig A) show that <i>ptpA</i> gene does not effect the cell growth. The biofilm formation of three strains in non-conductive 24-well plates(Fig B) and MFC anodes(Fig D). The ability for the mutant strain to form biofilm is reduced compared to the WT and complementaion in both surfaces. With less biofilm in the electrode, the mutant strain can create a higher current in MFC(Fig C), and reduce the MFC internal resistance(Fig E). | The growth curves of the wild-type(WT), ∆<i>ptpA</i> and the complementaion strain(Fig A) show that <i>ptpA</i> gene does not effect the cell growth. The biofilm formation of three strains in non-conductive 24-well plates(Fig B) and MFC anodes(Fig D). The ability for the mutant strain to form biofilm is reduced compared to the WT and complementaion in both surfaces. With less biofilm in the electrode, the mutant strain can create a higher current in MFC(Fig C), and reduce the MFC internal resistance(Fig E). | ||
Latest revision as of 12:39, 11 October 2023
Pci-RBS-ptpA-TT
Uses a promoter PcI(BBa_R0051) ,strong RBS(BBa_J34801), ptpA(BBa_K4767002)and TT(BBa_B0015). This part is an easy BioBrick.
Usage and Biology
This part is used to express ptpA gene in the mutant strain as a complementation to verify its function in the Shewanella oneidensis.
We created the ptpA knockout mutant and complementation strain to verify its function in the formation and conductivity of S. oneidensis MR-1 biofilm. Our results show that the ability of the mutant strain to form biofilm on both conductive (well plate) and non-conductive (anode) surfaces significantly reduced, whereas the complementation strain restored this capability. In microbial fuel cells(MFC), maximum voltage of the mutant strain raised about 36% compared to the wild type with the lower internal resistance In summary, we come to the conclusion that ptpA gene in S. oneidensis MR-1 may possibly effect the biofilm formation and it’s conductivity by meditating the polysaccharide biosynthesis.
The growth curves of the wild-type(WT), ∆ptpA and the complementaion strain(Fig A) show that ptpA gene does not effect the cell growth. The biofilm formation of three strains in non-conductive 24-well plates(Fig B) and MFC anodes(Fig D). The ability for the mutant strain to form biofilm is reduced compared to the WT and complementaion in both surfaces. With less biofilm in the electrode, the mutant strain can create a higher current in MFC(Fig C), and reduce the MFC internal resistance(Fig E).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]