Difference between revisions of "Part:BBa K4837000"
 
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Optimized the sequence for E. coli expression
 
Optimized the sequence for E. coli expression
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This plasmid optimized the sequence from white-rot fungus (<i> Phanerochaete chrysosporium</i>) for <i> E. coli </i> expression.
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The product illustrated below, namely lignin peroxidase (LiP), works together with a DNA duplex and another product from  BBa_K4837001 to form a "cascade enzyme system" for PFAs degradation.
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<a href='https://static.igem.wiki/teams/4837/wiki/parts-lip.png' target="_blank"><img src="https://static.igem.wiki/teams/4837/wiki/parts-lip.png" width="25%"/>
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LiP is conjugated with an azide-modified DNA oligo, which is illustrated below:
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<a href='https://static.igem.wiki/teams/4837/wiki/description-photo-3.png' target="_blank"><img src="https://static.igem.wiki/teams/4837/wiki/description-photo-3.png" width="35%"/>
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Finally, the "cascade enzyme system" formed when two DNA oligos in the conjugated products bound with each other by complementary base pairing principle, which is illustrated below:
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<a href='https://static.igem.wiki/teams/4837/wiki/description-photo-4.png' target="_blank"><img src="https://static.igem.wiki/teams/4837/wiki/description-photo-4.png" width="60%"/>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 08:41, 12 October 2023


Lignin peroxidase

Optimized the sequence for E. coli expression

This plasmid optimized the sequence from white-rot fungus ( Phanerochaete chrysosporium) for E. coli expression.


The product illustrated below, namely lignin peroxidase (LiP), works together with a DNA duplex and another product from BBa_K4837001 to form a "cascade enzyme system" for PFAs degradation.

LiP is conjugated with an azide-modified DNA oligo, which is illustrated below:

Finally, the "cascade enzyme system" formed when two DNA oligos in the conjugated products bound with each other by complementary base pairing principle, which is illustrated below:

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 126
    Illegal NheI site found at 1694
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 66
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 260
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2352