Difference between revisions of "Part:BBa K4800011"
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<p style="font-size: 150%; font-weight: bold;">Build: | <p style="font-size: 150%; font-weight: bold;">Build: | ||
− | <p><img src="https://static.igem.wiki/teams/4800/wiki/parts/donor.png" width=" | + | <p><center><img src="https://static.igem.wiki/teams/4800/wiki/parts/donor.png" width="40%" height="40%"></center></p> |
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− | <strong>Fig1. | + | <strong>Fig1.YcjQ Donor</strong> |
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<p>The genes at both end of the YcjQ gene were selected as the upper and downer homology arms, which were amplified separately, purified and recovered, then joined by overlap to obtain the YcjQ Donor. | <p>The genes at both end of the YcjQ gene were selected as the upper and downer homology arms, which were amplified separately, purified and recovered, then joined by overlap to obtain the YcjQ Donor. |
Latest revision as of 09:03, 11 October 2023
YcjQ Donor
YcjQ Donor is exogenous DNA used by cells as a template for homologous recombination repair (HDR) after SgRNA and Cas9 complexes have caused DNA double-strand breaks at the target site.
Build:
![](https://static.igem.wiki/teams/4800/wiki/parts/donor.png)
Fig1.YcjQ Donor
The genes at both end of the YcjQ gene were selected as the upper and downer homology arms, which were amplified separately, purified and recovered, then joined by overlap to obtain the YcjQ Donor.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Unknown
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1373