Difference between revisions of "Part:BBa K4800019"
 
(7 intermediate revisions by the same user not shown)
Line 3: Line 3:
 
<partinfo>BBa_K4800019 short</partinfo>
 
<partinfo>BBa_K4800019 short</partinfo>
 
<p>
 
<p>
−
We constructed EutM-SpyCatcher building blocks and attached itself covalently via isopeptide bond formation by the SpyTag-SpyCatcher system to the formed scaffolds，joining with enzyme elements to form a complex andimproving the stability of the enzyme element.
+
We constructed EutM-SpyCatcher building blocks and attached itself covalently via isopeptide bond formation by the SpyTag-SpyCatcher system to the formed scaffolds，joining with enzyme elements to form a complex and improving the stability of the enzyme element.
 +
 
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
 +
  
 
<!-- -->
 
<!-- -->
−
 
−
<html lang="en">
 
−
 
−
<head>
 
−
    <meta charset="UTF-8">
 
−
    <meta http-equiv="X-UA-Compatible" content="IE=edge">
 
−
    <meta name="viewport" content="width=device-width, initial-scale=1.0">
 
−
    <title>Document</title>
 
−
</head>
 
−
 
−
<body>
 
−
    <p style="font-size: 120%; font-weight: bold;">Build:
 
−
    </p >
 
−
<p> We amplified EutM-SpyCatcher-containing fragments from lab-conserved strains.</p >
 
−
 
−
</body>
 
−
 
−
</html>
 
−
 
  
  
 +
</p>
 
<!-- -->
 
<!-- -->
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 12:53, 12 October 2023


EutM-SpyCatcher

We constructed EutM-SpyCatcher building blocks and attached itself covalently via isopeptide bond formation by the SpyTag-SpyCatcher system to the formed scaffolds，joining with enzyme elements to form a complex and improving the stability of the enzyme element.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 42
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 42
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 42
    Illegal BamHI site found at 36
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 42
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 42
  • 1000
    COMPATIBLE WITH RFC[1000]