Difference between revisions of "Part:BBa K4960026"
 
(2 intermediate revisions by one other user not shown)
Line 5: Line 5:
  
 
===Profile===
 
===Profile===
−
Name: pvc13_NTD-1*GGGSG-CKGGRAKDC-1*GGGSG-pvc13_CTD<br>
+
Name: pvc13_NTD-1*GGSGG-CKGGRAKDC-1*GGSGG-pvc13_CTD<br>
 
Base Pairs: 1362bp<br>
 
Base Pairs: 1362bp<br>
 
Origin: Synthetic<br>
 
Origin: Synthetic<br>
Line 11: Line 11:
 
<!-- Add more about the biology of this part here-->
 
<!-- Add more about the biology of this part here-->
 
===Usage and Biology===
 
===Usage and Biology===
−
As an indispensable component of recombinant fusion proteins, linkers have shown increasing importance in the construction of bioactive fusion proteins.[1] (GGGGS)n is a commonly used flexible linker. Here, we use (GGGGS)1 to influence the structure of tail fiber connected to the CKGGRAKDC.
+
As an indispensable component of recombinant fusion proteins, linkers have shown increasing importance in the construction of bioactive fusion proteins.[1] (GGGGS)n is a commonly used flexible linker. Here, we use (GGGGS)1 to influence the structure of tail fiber connected to the CKGGRAKDC.('''Figure 1''')
 
<html>
 
<html>
  
 
<figure class="figure">
 
<figure class="figure">
−
<p><img src="https://static.igem.wiki/teams/4960/wiki/basic-part/pvc25-26-27-28-29/1.png" alt="" width="800" height="1000" /></p>
+
<p><img src="https://static.igem.wiki/teams/4960/wiki/basic-part/pvc25-26-27-28-29/1.png" alt="" width="900" height="1000" /></p>
  
 
</figure>
 
</figure>
  
 
</html>
 
</html>
−
Figure 1.Schematic diagram of the influence of different Linkers on the structure of tail fibers
+
'''Figure 1. AlphaFold2-guided Design of Adipose Cell-targeting PVC Coat Expressing Plasmids.'''  AlphaFold2 based prediction of engineered PVC tail fiber trimer structure. Structure of adipose-targeting CKGGRAKDC peptide-presenting PVC tail fiber with indicated linkers were shown.
−
<html>
+
−
 
+
−
<figure class="figure">
+
−
<p><img src="https://static.igem.wiki/teams/4960/wiki/basic-part/pvc25-26-27-28-29/4.png" alt="" width="800" height="1000" /></p>
+
−
 
+
−
</figure>
+
−
 
+
−
</html>
+
−
Figure 2.PVC illustration   
+
−
<html>
+
−
 
+
−
<figure class="figure">
+
−
<p><img src="https://static.igem.wiki/teams/4960/wiki/basic-part/pvc25-26-27-28-29/2.png" alt="" width="800" height="600" /></p>
+
−
 
+
−
</figure>
+
−
</html> 
+
−
Figure 3.Horizontal cutaway view of the fiber region of the syringe.
+
 
===Special Design===
 
===Special Design===
 
Use the flexible linker on both sides of the CKGGRAKDC to affect the structure and function of the tail protein.  
 
Use the flexible linker on both sides of the CKGGRAKDC to affect the structure and function of the tail protein.  

Latest revision as of 06:43, 12 October 2023

pvc13 NTD-1*GGSGG-CKGGRAKDC-1*GGSGG-pvc13 CTD

A flexible linker which can influence the structure of tail fiber connected to the CKGGRAKDC.

Profile

Name: pvc13_NTD-1*GGSGG-CKGGRAKDC-1*GGSGG-pvc13_CTD
Base Pairs: 1362bp
Origin: Synthetic
Properties: A flexible linker which can influence the structure of tail fiber connected to the CKGGRAKDC.

Usage and Biology

As an indispensable component of recombinant fusion proteins, linkers have shown increasing importance in the construction of bioactive fusion proteins.[1] (GGGGS)n is a commonly used flexible linker. Here, we use (GGGGS)1 to influence the structure of tail fiber connected to the CKGGRAKDC.(Figure 1)

Figure 1. AlphaFold2-guided Design of Adipose Cell-targeting PVC Coat Expressing Plasmids. AlphaFold2 based prediction of engineered PVC tail fiber trimer structure. Structure of adipose-targeting CKGGRAKDC peptide-presenting PVC tail fiber with indicated linkers were shown.

Special Design

Use the flexible linker on both sides of the CKGGRAKDC to affect the structure and function of the tail protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 309
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

[1] Chen, X., Zaro, J. L., & Shen, W. C. (2013). Fusion protein linkers: property, design and functionality. Advanced drug delivery reviews, 65(10), 1357–1369.