Difference between revisions of "Part:BBa K4960014"

 
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<partinfo>BBa_K4960014 short</partinfo>
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<partinfo>BBa_K4960014 short</partinfo><br>
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Pvc15 functions as tape measure protein and ATPase.
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===Profile===
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Name: PVC15<br>
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Base Pairs: 2097bp<br>
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Origin: <i>Photorhabdus</i><br>
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Properties: Pvc15 functions as tape measure protein and ATPase.<br>
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===Usage and Biology===
 
===Usage and Biology===
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Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices for targeted delivery of proteins .[1]Structurally similar to other CISs, PVCs are also macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2]('''Figure1''')<br>
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<img src="https://static.igem.wiki/teams/4960/wiki/basic-part/pvc1-16.jpg" class="figure-img img-fluid rounded"  height="150px">
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'''Figure 1. Schematic of the PVCpnf locus. '''It contains 16 structural and accessory genes , followed by two payload genes (Pdp1 and Pnf, in red) and four putative regulatory genes (in pink).<br>
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Pvc15 is present at very low levels in the PVC syringes sample and is absent in CISs like phage T4 or R-type pyocins.According to previous studies,it possibly functions as tape measure protein and ATPase.
  
 
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
<partinfo>BBa_K4960014 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4960014 SequenceAndFeatures</partinfo>
  
  
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===Functional Parameters===
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===References===
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<partinfo>BBa_K4960014 parameters</partinfo>
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[1]Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Programmable protein delivery with a bacterial contractile injection system. Nature. 2023 Apr;616(7956):357-364. doi: 10.1038/s41586-023-05870-7. Epub 2023 Mar 29. <br>
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[2]Jiang F, Li N, Wang X, Cheng J, Huang Y, Yang Y, Yang J, Cai B, Wang YP, Jin Q, Gao N. Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System. Cell. 2019 Apr 4;177(2):370-383.e15. doi: 10.1016/j.cell.2019.02.020. Epub 2019 Mar 21. <br>

Latest revision as of 14:08, 12 October 2023


pvc15
Pvc15 functions as tape measure protein and ATPase.

Profile

Name: PVC15
Base Pairs: 2097bp
Origin: Photorhabdus
Properties: Pvc15 functions as tape measure protein and ATPase.

Usage and Biology

Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices for targeted delivery of proteins .[1]Structurally similar to other CISs, PVCs are also macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2](Figure1)

Figure 1. Schematic of the PVCpnf locus. It contains 16 structural and accessory genes , followed by two payload genes (Pdp1 and Pnf, in red) and four putative regulatory genes (in pink).

Pvc15 is present at very low levels in the PVC syringes sample and is absent in CISs like phage T4 or R-type pyocins.According to previous studies,it possibly functions as tape measure protein and ATPase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 87
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 618
    Illegal NgoMIV site found at 757
    Illegal AgeI site found at 1056
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1]Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Programmable protein delivery with a bacterial contractile injection system. Nature. 2023 Apr;616(7956):357-364. doi: 10.1038/s41586-023-05870-7. Epub 2023 Mar 29.
[2]Jiang F, Li N, Wang X, Cheng J, Huang Y, Yang Y, Yang J, Cai B, Wang YP, Jin Q, Gao N. Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System. Cell. 2019 Apr 4;177(2):370-383.e15. doi: 10.1016/j.cell.2019.02.020. Epub 2019 Mar 21.