Difference between revisions of "Part:BBa K4960013"

 
(→‎Profile)
 
(4 intermediate revisions by 2 users not shown)
Line 1: Line 1:
  
 
__NOTOC__
 
__NOTOC__
−
<partinfo>BBa_K4960013 short</partinfo>
+
<partinfo>BBa_K4960013 short</partinfo><br>
 +
Pvc14 possibly controls tube optimal length.
 +
===Profile===
 +
Name: pvc14<br>
 +
Base Pairs: 1716bp<br>
 +
Origin: <i>Photorhabdus</i><br>
 +
Properties: Pvc14 possibly controls tube optimal length<br>
  
−
a
+
<!-- Add more about the biology of this part here-->
  
−
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices for targeted delivery of proteins .[1]Structurally similar to other CISs, PVCs are also macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2]('''Figure1''')<br>
 +
<html>
  
 +
<figure class="figure">
 +
<img src="https://static.igem.wiki/teams/4960/wiki/basic-part/pvc1-16.jpg" class="figure-img img-fluid rounded"  height="150px">
 +
 +
</figure>
 +
 +
</html>
 +
'''Figure 1. Schematic of the PVCpnf locus.''' It contains 16 structural and accessory genes , followed by two payload genes (Pdp1 and Pnf, in red) and four putative regulatory genes (in pink).<br>
 +
<br>
 +
Pvc14 is a homolog of Afp14 in Serratia Afp, which was proposed to act as a tape measure protein.According to previous studies,it is present at very low levels in the PVC syringes sample.Significantly,its deletion resulted in a large variation in the length of PVC particles.Therefore,it possibly controls tube optimal length.<br>
 +
<br>
 
<!-- -->
 
<!-- -->
−
<span class='h3bb'>Sequence and Features</span>
+
===Sequence and Features===
 
<partinfo>BBa_K4960013 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4960013 SequenceAndFeatures</partinfo>
  
  
−
<!-- Uncomment this to enable Functional Parameter display  
+
<!-- Uncomment this to enable Functional Parameter display -->
−
===Functional Parameters===
+
===References===
−
<partinfo>BBa_K4960013 parameters</partinfo>
+
[1]Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Programmable protein delivery with a bacterial contractile injection system. Nature. 2023 Apr;616(7956):357-364. doi: 10.1038/s41586-023-05870-7. Epub 2023 Mar 29. <br>
−
<!-- -->
+
[2]Jiang F, Li N, Wang X, Cheng J, Huang Y, Yang Y, Yang J, Cai B, Wang YP, Jin Q, Gao N. Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System. Cell. 2019 Apr 4;177(2):370-383.e15. doi: 10.1016/j.cell.2019.02.020. Epub 2019 Mar 21. <br>

Latest revision as of 14:08, 12 October 2023


pvc14
Pvc14 possibly controls tube optimal length.

Profile

Name: pvc14
Base Pairs: 1716bp
Origin: Photorhabdus
Properties: Pvc14 possibly controls tube optimal length


Usage and Biology

Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices for targeted delivery of proteins .[1]Structurally similar to other CISs, PVCs are also macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2](Figure1)

Figure 1. Schematic of the PVCpnf locus. It contains 16 structural and accessory genes , followed by two payload genes (Pdp1 and Pnf, in red) and four putative regulatory genes (in pink).

Pvc14 is a homolog of Afp14 in Serratia Afp, which was proposed to act as a tape measure protein.According to previous studies,it is present at very low levels in the PVC syringes sample.Significantly,its deletion resulted in a large variation in the length of PVC particles.Therefore,it possibly controls tube optimal length.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1]Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Programmable protein delivery with a bacterial contractile injection system. Nature. 2023 Apr;616(7956):357-364. doi: 10.1038/s41586-023-05870-7. Epub 2023 Mar 29.
[2]Jiang F, Li N, Wang X, Cheng J, Huang Y, Yang Y, Yang J, Cai B, Wang YP, Jin Q, Gao N. Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System. Cell. 2019 Apr 4;177(2):370-383.e15. doi: 10.1016/j.cell.2019.02.020. Epub 2019 Mar 21.