Difference between revisions of "Part:BBa K4960002"
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__NOTOC__
 
__NOTOC__
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<partinfo>BBa_K4960002 short</partinfo>
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<partinfo>BBa_K4960002 short</partinfo><br>
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===Properties===
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Pvc2 is a part of the sheath
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===Profile===
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Name: pvc2<br>
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Base Pairs: 1068 bp<br>
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Origin: <i>Photorhabdus</i><br>
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Properties: Pvc2 is a part of the sheath.<br>
  
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Pvc2 is a part of the sheath.
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<!-- Add more about the biology of this part here-->
  
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<!-- Add more about the biology of this part here-->
 
 
===Usage and Biology===
 
===Usage and Biology===
  
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Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices and present in both bacteria and archaea.[1]In structure, PVCs are macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2](Figure1,Figure2)<br>
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Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices and present in both bacteria and archaea.[1]In structure, PVCs are macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2]('''Figure1''')<br>
 
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<figure class="figure">
 
<figure class="figure">
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<img src="https://static.igem.wiki/teams/4960/wiki/basic-part/pvc2-3-4-5-6-7.png" class="figure-img img-fluid rounded"  height="200px">
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<img src="https://static.igem.wiki/teams/4960/wiki/basic-part/pvc1-16.jpg" class="figure-img img-fluid rounded"  height="150px">
  
 
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Figure1:PVC illustrations<br>
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'''Figure 1. Schematic of the PVCpnf locus.''' It contains 16 structural and accessory genes , followed by two payload genes (Pdp1 and Pnf, in red) and four putative regulatory genes (in pink).<br>
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Figure2:Payload illustrations<br>
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<br>
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The sheath in the pre-contraction state is composed of 23 stacked hexameric rings of sheath proteins, Pvc4, Pvc3, and Pvc2. A part of sheath, comprises alternating Pvc2 and Pvc3 subunits, which both interact with Pvc1. The PVC sheath body is predominantly made of Pvc2 hexamers,and deletion of the main sheath protein Pvc2 results in sheath-free particles. <br>
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The sheath in the pre-contraction state is composed of 23 stacked hexameric rings of sheath proteins, Pvc4, Pvc3, and Pvc2. A part of sheath, comprises alternating Pvc2 and Pvc3 subunits, which both interact with Pvc1. The PVC sheath body is predominantly made of Pvc2 hexamers,(Figure.3) and deletion of the main sheath protein Pvc2 results in sheath-free particles. <br>
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Among the three, Pvc2 has the simplest structure, which closely relates to the R-type pyocin sheath protein. Pvc2 consists of two major domains (domain I and II) <br>
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Among the three, Pvc2 has the simplest structure, which closely relates to the R-type pyocin sheath protein. Pvc2 is consisted of two major domains (domain I and II) <br>
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Pvc2 starts to assemble along the inner tube for the maturation of PVC particles with Pvc3 at the end of assembly.<br>
 
Pvc2 starts to assemble along the inner tube for the maturation of PVC particles with Pvc3 at the end of assembly.<br>
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<html>(Figure.4)
 
  
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<figure class="figure">
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===Sequence and Features===
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<img src="https://static.igem.wiki/teams/4960/wiki/basic-part/pvc2-2.png" class="figure-img img-fluid rounded"  height="200px">
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<img src="https://static.igem.wiki/teams/4960/wiki/basic-part/pvc2.png" class="figure-img img-fluid rounded"  height="200px">
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<img src="https://static.igem.wiki/teams/4960/wiki/basic-part/pvc-2-4.png" class="figure-img img-fluid rounded"  height="200px">
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</figure>
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</html>
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Figure3.The proteins consisting of the pvc<br>
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Figure4.The protein pvc2 encodes
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4960002 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4960002 SequenceAndFeatures</partinfo>
  
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<partinfo>BBa_K4960002 parameters</partinfo>
 
<partinfo>BBa_K4960002 parameters</partinfo>
 
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===Reference===
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===References===
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[1]Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Programmable protein delivery with a bacterial contractile injection system. Nature. 2023 Apr;<br>
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[1]Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Programmable protein delivery with a bacterial contractile injection system. Nature. 2023 Apr;616(7956):357-364. doi: 10.1038/s41586-023-05870-7. Epub 2023 Mar 29. <br>
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[2]Jiang F, Li N, Wang X, Cheng J, Huang Y, Yang Y, Yang J, Cai B, Wang YP, Jin Q, Gao N. Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System. Cell. 2019 Apr
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[2]Jiang F, Li N, Wang X, Cheng J, Huang Y, Yang Y, Yang J, Cai B, Wang YP, Jin Q, Gao N. Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System. Cell. 2019 Apr 4;177(2):370-383.e15. doi: 10.1016/j.cell.2019.02.020. Epub 2019 Mar 21. <br>

Latest revision as of 14:02, 12 October 2023


pvc2
Pvc2 is a part of the sheath

Profile

Name: pvc2
Base Pairs: 1068 bp
Origin: Photorhabdus
Properties: Pvc2 is a part of the sheath.


Usage and Biology

Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices and present in both bacteria and archaea.[1]In structure, PVCs are macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2](Figure1)

Figure 1. Schematic of the PVCpnf locus. It contains 16 structural and accessory genes , followed by two payload genes (Pdp1 and Pnf, in red) and four putative regulatory genes (in pink).

The sheath in the pre-contraction state is composed of 23 stacked hexameric rings of sheath proteins, Pvc4, Pvc3, and Pvc2. A part of sheath, comprises alternating Pvc2 and Pvc3 subunits, which both interact with Pvc1. The PVC sheath body is predominantly made of Pvc2 hexamers,and deletion of the main sheath protein Pvc2 results in sheath-free particles.
Among the three, Pvc2 has the simplest structure, which closely relates to the R-type pyocin sheath protein. Pvc2 consists of two major domains (domain I and II)
Pvc2 starts to assemble along the inner tube for the maturation of PVC particles with Pvc3 at the end of assembly.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1]Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Programmable protein delivery with a bacterial contractile injection system. Nature. 2023 Apr;616(7956):357-364. doi: 10.1038/s41586-023-05870-7. Epub 2023 Mar 29.
[2]Jiang F, Li N, Wang X, Cheng J, Huang Y, Yang Y, Yang J, Cai B, Wang YP, Jin Q, Gao N. Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System. Cell. 2019 Apr 4;177(2):370-383.e15. doi: 10.1016/j.cell.2019.02.020. Epub 2019 Mar 21.