Difference between revisions of "Part:BBa K4724076"
 
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<partinfo>BBa_K4724076 short</partinfo>
 
<partinfo>BBa_K4724076 short</partinfo>
  
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We fused the NusA tag to the N-terminus of LSPETase to enhance its folding characteristics and increase the solubility of the recombinant protein, thereby reducing the formation of inclusion bodies.
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We fused the NusA tag to the N-terminus of LSPETase to enhance its folding characteristics and increase the solubility of the recombinant protein, thereby reducing the formation of inclusion bodies.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 19:28, 10 October 2023


TrxA-LSPETase

We fused the NusA tag to the N-terminus of LSPETase to enhance its folding characteristics and increase the solubility of the recombinant protein, thereby reducing the formation of inclusion bodies.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 25