Difference between revisions of "Part:BBa K4724075:Design"

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<partinfo>BBa_K4724075 short</partinfo>
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<h2 style="font-weight:600">NusA-LSPET </h2>
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<partinfo>BBa_K4724075 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4724075 SequenceAndFeatures</partinfo>
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===Design Notes===
 
===Design Notes===
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A recombinant plasmid constructed using the Gibson assembly method was inserted into the vector pET-22b(+)-LSPET-12
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The DNA of LSPETase and NusA were both inserted into a plasmid, resulting in the construction of a recombinant plasmid. Subsequently, transcription and translation were carried out, leading to the fusion of NusA at the N-terminus of LSPETase.
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===Source===
 
===Source===

Latest revision as of 19:23, 10 October 2023


NusA-LSPET


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1368
    Illegal XhoI site found at 2344
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1600
    Illegal AgeI site found at 1687
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The DNA of LSPETase and NusA were both inserted into a plasmid, resulting in the construction of a recombinant plasmid. Subsequently, transcription and translation were carried out, leading to the fusion of NusA at the N-terminus of LSPETase.

Source

Laboratory design in our research group.

References