Difference between revisions of "Part:BBa K4800041"
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<partinfo>BBa_K4800041 short</partinfo> | <partinfo>BBa_K4800041 short</partinfo> | ||
− | + | We linearized the pTrc99a vector using restriction endonuclease and connected the target gene PatA to it through homologous recombination to obtain the recombinant plasmid pTrc99a-PatA. | |
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===Usage and Biology=== | ===Usage and Biology=== | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> |
Latest revision as of 08:41, 11 October 2023
pTrc99a-PatA
We linearized the pTrc99a vector using restriction endonuclease and connected the target gene PatA to it through homologous recombination to obtain the recombinant plasmid pTrc99a-PatA.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 4036
Illegal PstI site found at 4048 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 4048
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 4030
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 4036
Illegal PstI site found at 4048 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 4036
Illegal PstI site found at 4048
Illegal NgoMIV site found at 2804
Illegal AgeI site found at 3311 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 4232
Illegal BsaI site found at 5300
Illegal SapI.rc site found at 767