Difference between revisions of "Part:BBa K4630100:Design"

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                 <p style="color:Gray; padding:0px 30px 10px;">Figure 1. Design of stgRNA-barcode-cassette 1</p>
 
                 <p style="color:Gray; padding:0px 30px 10px;">Figure 1. Design of stgRNA-barcode-cassette 1</p>
 
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Latest revision as of 16:52, 10 October 2023


stgRNA-barcode-cassette 1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 55
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 94


Design Notes

As the first cassette, it contains Lac promoter to initiate the induced edition in the proof-of-concept. Also, the double terminator prevents it from expressing the next cassette. The barcode is also generated by the in-silico generator, to avoid recombination with the bacterial genome.

Figure 1. Design of stgRNA-barcode-cassette 1

Source

Complete synthesis.

References