Difference between revisions of "Part:BBa K4830001:Design"
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===Design Notes===
 
===Design Notes===
 
The design of RNF2 ngRNA includes the U6 promoter, spacer, guide RNA scaffold, and polyA tail.
 
The design of RNF2 ngRNA includes the U6 promoter, spacer, guide RNA scaffold, and polyA tail.
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===Source===
 
===Source===
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The original RNF2 ngRNA design containing spacer sequence was reported in Prime Editor 2 paper cited in References. We used that sequence and with the help of and TWIST Bioscience synthesized it, which was further pieced together with 65777 backbone in our lab.
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The original RNF2 ngRNA design containing spacer sequence was reported in Prime Editor 2 paper cited in References. With the help of TWIST Bioscience we synthesized that sequence and further pieced together with 65777 backbone in our lab.
  
 
===References===
 
===References===
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.Anzalone AV, Randolph PB, Davis JR, Sousa AA, Koblan LW, Levy JM, et al. Search-and-replace genome editing without double-strand breaks or donor DNA. Nature. 2019 Oct 21;576.
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Anzalone AV, Randolph PB, Davis JR, Sousa AA, Koblan LW, Levy JM, et al. Search-and-replace genome editing without double-strand breaks or donor DNA. Nature. 2019 Oct 21;576.

Latest revision as of 16:15, 10 October 2023


RNF ngRNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The design of RNF2 ngRNA includes the U6 promoter, spacer, guide RNA scaffold, and polyA tail.

Source

The original RNF2 ngRNA design containing spacer sequence was reported in Prime Editor 2 paper cited in References. With the help of TWIST Bioscience we synthesized that sequence and further pieced together with 65777 backbone in our lab.

References

Anzalone AV, Randolph PB, Davis JR, Sousa AA, Koblan LW, Levy JM, et al. Search-and-replace genome editing without double-strand breaks or donor DNA. Nature. 2019 Oct 21;576.