Difference between revisions of "Part:BBa K4879000:Design"

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===Design Notes===
 
===Design Notes===
−
The coding sequence has been codon-optimized for <i>Y. lipolytica</i>. It also has silent mutations to make the part Type II Standard compatible.  
+
The coding sequence has been codon-optimized for <i>Y. lipolytica</i> using SnapGene. It also has silent mutations to make the part Type II Standard compatible.  
  
  
  
 
===Source===
 
===Source===
−
The part had been synthesized by Integrated DNA Technologies, Inc.
+
The part had been synthesized by Twist Bioscience, iGEM's DNA synthesis partner.
  
 +
https://www.ncbi.nlm.nih.gov/gene/105644367
 
===References===
 
===References===
 
Liu, Y.; Han, J.; Li, Z.; Jiang, Z.; Luo, L.; Zhang, Y.; Chen, M.; Yang, Y.; Liu, Z. Heterologous Expression of Jatropha curcas Fatty Acyl-ACP Thioesterase A (JcFATA) and B (JcFATB) Affects Fatty Acid Accumulation and Promotes Plant Growth and Development in Arabidopsis. Int. J. Mol. Sci. 2022, 23, 4209. https://doi.org/10.3390/ijms23084209
 
Liu, Y.; Han, J.; Li, Z.; Jiang, Z.; Luo, L.; Zhang, Y.; Chen, M.; Yang, Y.; Liu, Z. Heterologous Expression of Jatropha curcas Fatty Acyl-ACP Thioesterase A (JcFATA) and B (JcFATB) Affects Fatty Acid Accumulation and Promotes Plant Growth and Development in Arabidopsis. Int. J. Mol. Sci. 2022, 23, 4209. https://doi.org/10.3390/ijms23084209

Latest revision as of 12:42, 11 October 2023


JcFATA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 331
    Illegal PstI site found at 938
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 331
    Illegal PstI site found at 938
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 780
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 331
    Illegal PstI site found at 938
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 331
    Illegal PstI site found at 938
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The coding sequence has been codon-optimized for Y. lipolytica using SnapGene. It also has silent mutations to make the part Type II Standard compatible.


Source

The part had been synthesized by Twist Bioscience, iGEM's DNA synthesis partner.

https://www.ncbi.nlm.nih.gov/gene/105644367

References

Liu, Y.; Han, J.; Li, Z.; Jiang, Z.; Luo, L.; Zhang, Y.; Chen, M.; Yang, Y.; Liu, Z. Heterologous Expression of Jatropha curcas Fatty Acyl-ACP Thioesterase A (JcFATA) and B (JcFATB) Affects Fatty Acid Accumulation and Promotes Plant Growth and Development in Arabidopsis. Int. J. Mol. Sci. 2022, 23, 4209. https://doi.org/10.3390/ijms23084209