Difference between revisions of "Part:BBa K4960022:Design"

 
(→‎Source)
 
Line 15: Line 15:
 
===Source===
 
===Source===
  
−
Photorhabdus, synthetic
+
Photorhabdus, Homo Sapiens, Aequorea Victoria
  
 
===References===
 
===References===

Latest revision as of 16:11, 11 October 2023


Pdp1NTD-3*GGSGG-UCP1-2*GGSGG-EGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 505
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 235
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1.The linker which contains three repeating amino acid sequences links the packaging domain and another DNA fragment. After gene transcription and translation, in protein structures, linker sequences are usually flexible because the domains they connect to often have different conformation and motor requirements. Therefore, linker sequences often act as flexible connections, allowing the domain to move freely while maintaining structural stability. 2.The DNA fragment containing two BsaⅠ restriction sites opens up the possibility of subsequent connections to protein-coding genes. Sequence and Feature


Source

Photorhabdus, Homo Sapiens, Aequorea Victoria

References