Difference between revisions of "Part:BBa K4613508"

 
 
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This composite part contains BBa_J64997, BBa_K592016, BBa_B0053, BBa_K2277233, BBa_K4613453, and BBa_E0040, which was used as a control to minimize the effect of variables and motif 58.16.
 
This composite part contains BBa_J64997, BBa_K592016, BBa_B0053, BBa_K2277233, BBa_K4613453, and BBa_E0040, which was used as a control to minimize the effect of variables and motif 58.16.
  
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==== Reference ====
 +
#Ranzani A T, Wehrmann M, Kaiser J, et al. Light-dependent control of bacterial expression at the mRNA level[J]. ACS Synthetic Biology, 2022, 11(10): 3482-3492.
 +
#Weber A M, Kaiser J, Ziegler T, et al. A blue light receptor that mediates RNA binding and translational regulation[J]. Nature chemical biology, 2019, 15(11): 1085-1092.
 +
#Dietler J, Gelfert R, Kaiser J, et al. Signal transduction in light-oxygen-voltage receptors lacking the active-site glutamine[J]. Nature Communications, 2022, 13(1): 2618.
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 19:45, 10 October 2023


YF1-Motif_58.16-GFP

This composite part contains BBa_J64997, BBa_K592016, BBa_B0053, BBa_K2277233, BBa_K4613453, and BBa_E0040, which was used as a control to minimize the effect of variables and motif 58.16.

Reference

  1. Ranzani A T, Wehrmann M, Kaiser J, et al. Light-dependent control of bacterial expression at the mRNA level[J]. ACS Synthetic Biology, 2022, 11(10): 3482-3492.
  2. Weber A M, Kaiser J, Ziegler T, et al. A blue light receptor that mediates RNA binding and translational regulation[J]. Nature chemical biology, 2019, 15(11): 1085-1092.
  3. Dietler J, Gelfert R, Kaiser J, et al. Signal transduction in light-oxygen-voltage receptors lacking the active-site glutamine[J]. Nature Communications, 2022, 13(1): 2618.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 581
    Illegal NgoMIV site found at 653
    Illegal NgoMIV site found at 743
    Illegal NgoMIV site found at 761
    Illegal NgoMIV site found at 1273
    Illegal NgoMIV site found at 1566
    Illegal NgoMIV site found at 1660
    Illegal AgeI site found at 295
    Illegal AgeI site found at 1441
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1330
    Illegal BsaI.rc site found at 194
    Illegal BsaI.rc site found at 2818