Difference between revisions of "Part:BBa K4724023:Design"

(→‎Design Notes)
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===Source===
 
===Source===
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Designed by NJTech-China-A.
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We use Linker to fuse the domain to the <i>Is</i>PETase protein and connect 6xHis Tags behind the domain for easy purification.
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===References===
 
===References===

Latest revision as of 14:09, 10 October 2023


IsPETase-Linker-CBM11-6xHisTag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 56
    Illegal PstI site found at 937
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 56
    Illegal PstI site found at 937
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 790
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 56
    Illegal PstI site found at 937
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 56
    Illegal PstI site found at 937
    Illegal AgeI site found at 546
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We use Linker to fuse the domain to the IsPETase protein and connect 6xHis Tags behind the domain for easy purification.

Source

Designed by NJTech-China-A.

References