Difference between revisions of "Part:BBa K4724023:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | We use Linker to fuse the domain to the <i>Is</i>PETase protein and connect 6xHis Tags behind the domain for easy purification. | |
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===Source=== | ===Source=== | ||
| − | + | Designed by NJTech-China-A. | |
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===References=== | ===References=== | ||
Latest revision as of 14:09, 10 October 2023
IsPETase-Linker-CBM11-6xHisTag
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 56
Illegal PstI site found at 937 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 56
Illegal PstI site found at 937 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 790
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 56
Illegal PstI site found at 937 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 56
Illegal PstI site found at 937
Illegal AgeI site found at 546 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We use Linker to fuse the domain to the IsPETase protein and connect 6xHis Tags behind the domain for easy purification.
Source
Designed by NJTech-China-A.