Difference between revisions of "Part:BBa K243008:Design"

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===Design Notes===
 
===Design Notes===
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The length of the linker is important to guarantee the independent function of the protein domain when it is linked two another part.<br>
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The middle length of the linker is useful to produce a stable connection to another part and to create a certain distance. The middle distance produced by the linker reduces possible sterical interferences and increases the distance from the protein Fok_a to the DNA when it is linked to a DNA binding protein. We had to prove the choice of distance between two parts and the distance between our protein domains Fok_a and Fok_i.       <br>
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Designed according the assembly standard 25 [https://parts.igem.org/Assembly_standard_25 RFC 25]
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Designed according to the [https://parts.igem.org/Assembly_standard_25 RFC 25].
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[https://static.igem.org/mediawiki/parts/f/f3/Freiburg09_ML-FokA.txt Commented GenBank file]
  
 
===Source===
 
===Source===

Latest revision as of 00:53, 22 October 2009

Middle Linker-Fok_a


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 517


Design Notes

The middle length of the linker is useful to produce a stable connection to another part and to create a certain distance. The middle distance produced by the linker reduces possible sterical interferences and increases the distance from the protein Fok_a to the DNA when it is linked to a DNA binding protein. We had to prove the choice of distance between two parts and the distance between our protein domains Fok_a and Fok_i.
Designed according to the RFC 25.

Commented GenBank file

Source

Combinated by cloning.

References