Difference between revisions of "Part:BBa K243005:Design"

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===Design Notes===
 
===Design Notes===
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Designed for the fusion of proteins or peptides via in frame cloning, according to RFC25.<br>
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Glycine and serine are very suitable for repetitive sequences. Their properties zwitterionic and hydrophile prevent aggregation of the linker.<br>
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The sequence has no cleavage site for proteases. <br>
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[[Image:Freiburg09 Middlelipart.png|750x550px]] <br>
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Designed for the fusion of proteins or peptides via in frame cloning, according to [https://parts.igem.org/Assembly_standard_25 RFC 25].<br>
 
[https://static.igem.org/mediawiki/parts/b/bd/MiddleLinker.txt Commented GenBank file]
 
[https://static.igem.org/mediawiki/parts/b/bd/MiddleLinker.txt Commented GenBank file]
  
 
===Source===
 
===Source===
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Oligonucleotides designed by Team Freiburg Bioware09 and synthesized by sigma. Hybridized by PCR.
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Oligos synthesized by sigma. Hybridized by PCR.  
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===References===
 
===References===

Latest revision as of 20:48, 21 October 2009

Middle Linker ( Gly-Gly-Ser-Gly)x2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Glycine and serine are very suitable for repetitive sequences. Their properties zwitterionic and hydrophile prevent aggregation of the linker.
The sequence has no cleavage site for proteases.
Freiburg09 Middlelipart.png
Designed for the fusion of proteins or peptides via in frame cloning, according to RFC 25.
Commented GenBank file

Source

Oligonucleotides designed by Team Freiburg Bioware09 and synthesized by sigma. Hybridized by PCR.

References