Difference between revisions of "Part:BBa K243003:Design"

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===Design Notes===
 
===Design Notes===
−
Freiburg standard25 part
+
Designed with Biobrick pre-and suffix for fusion proteins according to the [https://parts.igem.org/Assembly_standard_25 RFC 25].<br>
 +
[https://parts.igem.org/Image:DigA.txt  Commented GenBank file]
  
 
===Source===
 
===Source===
  
−
synthesized by purimex.<br>
+
Planned and designed by Team Freiburg Bioware09 and synthesized by purimex.<br>
−
[https://parts.igem.org/Image:DigA.txt  "Commented GenBank file"]
+
  
 
===References===
 
===References===
−
Holtke HJ, Ankenbauer W, Muhlegger K, Rein R, Sagner G, Seibl R, Walter T. (1995)
+
Holtke HJ, Ankenbauer W, Muhlegger K, Rein R, Sagner G, Seibl R, Walter T. (1995)<br>
−
The digoxigenin (DIG) system for non-radioactive labelling and detection of nucleic  
+
''The digoxigenin (DIG) system for non-radioactive labelling and detection of nucleic  
−
acids--an overview. Cell Mol Biol. 41(7):883-905.  
+
acids--an overview.'' Cell Mol Biol. 41(7):883-905.<br>
−
Roche Applied Science (2002) DIG Application Manual for Nonradioactive In Situ  
+
−
Hybridization (3rd
+
Roche Applied Science (2002) ''DIG Application Manual for Nonradioactive In Situ  
−
edition)  
+
Hybridization'' (3rdedition) <br>
−
Moter A, Göbel UB (2000) Fluorescence in situ hybridisation (FISH) for direct  
+
 
−
visualization of microorganism, J Microbiol Meth. 41: 85-112
+
Moter A, Göbel UB (2000) ''Fluorescence in situ hybridisation (FISH) for direct  
 +
visualization of microorganism'', J Microbiol Meth. 41: 85-112
 +
 
 +
http://www.thermo.com/eThermo/CMA/PDFs/Various/File_29495.pdf (image)

Latest revision as of 20:44, 21 October 2009

Digoxigenin binding protein (DigA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 248
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designed with Biobrick pre-and suffix for fusion proteins according to the RFC 25.
Commented GenBank file

Source

Planned and designed by Team Freiburg Bioware09 and synthesized by purimex.

References

Holtke HJ, Ankenbauer W, Muhlegger K, Rein R, Sagner G, Seibl R, Walter T. (1995)
The digoxigenin (DIG) system for non-radioactive labelling and detection of nucleic acids--an overview. Cell Mol Biol. 41(7):883-905.

Roche Applied Science (2002) DIG Application Manual for Nonradioactive In Situ Hybridization (3rdedition)

Moter A, Göbel UB (2000) Fluorescence in situ hybridisation (FISH) for direct visualization of microorganism, J Microbiol Meth. 41: 85-112

http://www.thermo.com/eThermo/CMA/PDFs/Various/File_29495.pdf (image)