Difference between revisions of "Part:BBa K4743004"
 
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We use electrophoresis to check the length of the fragment
 
We use electrophoresis to check the length of the fragment
  
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<html><img style="width:800px" src="https://static.igem.wiki/teams/4743/wiki/folder-for-parts/nade-legth-check.png" </html>
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<html><img style="width:800px" src="https://static.igem.wiki/teams/4743/wiki/folder-for-parts/nada-nadb-nade-length.png" </html>
  
 
<center>Figure 1. The length of NadE.</center>
 
<center>Figure 1. The length of NadE.</center>

Latest revision as of 07:40, 11 October 2023


NadE+ADH1 terminator with adaptor

NadE is an NAD synthase. According to William B. Black et al. , this synthase is also used in turning NaMN into NMN.

Length checking of nadE

We use electrophoresis to check the length of the fragment

Figure 1. The length of NadE.

Overall metabolic pathway of NMN

NadE is involved in the pathway of Astaxanthin production in PTSH-Taiwan's project.

Figure 2. The Matabolic pathway of NMN production.

References

1. Hughes, K. T., Olivera, B. M., & Roth, J. R. (1988). Structural gene for NAD synthetase in Salmonella typhimurium. Journal of bacteriology, 170(5), 2113–2120. https://doi.org/10.1128/jb.170.5.2113-2120.1988


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1052
    Illegal SpeI site found at 1045
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1052
    Illegal NheI site found at 747
    Illegal NheI site found at 823
    Illegal SpeI site found at 1045
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1052
    Illegal BamHI site found at 16
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1052
    Illegal SpeI site found at 1045
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1052
    Illegal SpeI site found at 1045
    Illegal AgeI site found at 175
    Illegal AgeI site found at 1065
  • 1000
    COMPATIBLE WITH RFC[1000]