Difference between revisions of "Part:BBa K4630114:Design"

 
 
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===Design Notes===
 
===Design Notes===
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Using Biobrick Assembly, the stgRNA-cassette (1+2) and stgRNA-cassette 3 can be ligated.  
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Using Biobrick Assembly, we introduced an XbaI site upstream of the segment to be inserted, and SpeI & EcoRI downstream of each cassette. By cutting the backbone with SpeI and EcoRI and cutting the segment with XbaI and EcoRI, the new cassette could be inserted downstream of the previous one(s). Besides, the remaining restriction sites make the system fully extendable to add additional cassettes.
  
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Finally, the stgRNA-cassette (1+2) and stgRNA-cassette 3 can be ligated to form the BBa K4630114.
  
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===Source===
 
===Source===

Latest revision as of 22:58, 11 October 2023


stgRNA-cassette (1+2+3)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Using Biobrick Assembly, we introduced an XbaI site upstream of the segment to be inserted, and SpeI & EcoRI downstream of each cassette. By cutting the backbone with SpeI and EcoRI and cutting the segment with XbaI and EcoRI, the new cassette could be inserted downstream of the previous one(s). Besides, the remaining restriction sites make the system fully extendable to add additional cassettes.

Finally, the stgRNA-cassette (1+2) and stgRNA-cassette 3 can be ligated to form the BBa K4630114.

Source

Complete synthesis.

References