Difference between revisions of "Part:BBa K177038"
 
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===Usage and Biology===
 
===Usage and Biology===
  
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The state of the switch is set either by IPTG or temperature. In 0,1 uM IPTG lacI gets inactivated ant transcription from Plac starts. It results in production of cIts and other genes belonging to this part of switch (in this case GFP). After removal of IPTG the state remains stable - P<sub>Rm</sub> is repressed by cIts and genes under Plac are expressed. In 42<sup>o</sup>C cIts gets inactivated and expression of genes under P<sub>Rm</sub> (in this case lacI and RFP) are expressed. Because lacI is expressed to Plac gets repressed. The state remains stable after transfer of this device to 30<sub>o</sub>C. State of the device can be switched by pulses of IPTG and 42<sub>o</sub>C. Temperature of stable work is 30<sub>o</sub>C.
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The state of the switch is set either by IPTG or temperature. In 0,1 uM IPTG lacI gets inactivated ant transcription from Plac starts. It results in production of cIts and other genes belonging to this part of switch (in this case GFP). After removal of IPTG the state remains stable - P<sub>Rm</sub> is repressed by cIts and genes under Plac are expressed. In 42<sup>o</sup>C cIts gets inactivated and genes under P<sub>Rm</sub> (in this case lacI and RFP) are expressed. Because lacI is expressed to Plac gets repressed. The state remains stable after transfer of this device to 30<sup>o</sup>C. State of the device can be switched by pulses of IPTG and 42<sup>o</sup>C. Temperature of stable work is 30<sup>o</sup>C.
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Please check http://2009.igem.org/Team:Warsaw/results for more informations.
  
 
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Latest revision as of 01:10, 22 October 2009

Bistable switch 1 testing device

Bistable switch based on termosensitive lambda repressor (cIts) and lactose operon inhibitor (lacI).


Usage and Biology

The state of the switch is set either by IPTG or temperature. In 0,1 uM IPTG lacI gets inactivated ant transcription from Plac starts. It results in production of cIts and other genes belonging to this part of switch (in this case GFP). After removal of IPTG the state remains stable - PRm is repressed by cIts and genes under Plac are expressed. In 42oC cIts gets inactivated and genes under PRm (in this case lacI and RFP) are expressed. Because lacI is expressed to Plac gets repressed. The state remains stable after transfer of this device to 30oC. State of the device can be switched by pulses of IPTG and 42oC. Temperature of stable work is 30oC.

Please check http://2009.igem.org/Team:Warsaw/results for more informations.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3058
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3645
    Illegal AgeI site found at 3757
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1614