Difference between revisions of "Part:BBa K188713"

Latest revision as of 14:12, 20 October 2009

GFP regulated by tetracycline, weaker GFP expression.

This device modify from BBa_K145279,designed by iGEM08_KULeuven. It consists if a TetR generator and a GFP under the control of a TetR regulated promoter. Normally the production of GFP is switched off. It can be switched on by the addition of tetracycline (or anhydrotetracycline ). This modified device use Degenerated PCR to make different TATA box at -10 sequences. This composite is similar to BBa_K188707, but the GFP strength is lighter than BBa_K188707.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1616

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K188713 short</partinfo>
-This device modify from BBa_K145279,design by iGEM08_KULeuven. It consists if a TetR generator and a GFP under the control of a TetR regulated promoter. Normally the production of GFP is switched off. It can be switched on by the addition of tetracycline (or anhydrotetracycline ). This modified device use Degenerated PCR to make different TATA box at -10 sequences. This composite is similar to BBa_K188707, but the GFP strength is lighter than BBa_K188707.
+This device modify from BBa_K145279,designed by iGEM08_KULeuven. It consists if a TetR generator and a GFP under the control of a TetR regulated promoter. Normally the production of GFP is switched off. It can be switched on by the addition of tetracycline (or anhydrotetracycline ). This modified device use Degenerated PCR to make different TATA box at -10 sequences. This composite is similar to BBa_K188707, but the GFP strength is lighter than BBa_K188707.
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