Difference between revisions of "Part:BBa K200027"
 
(One intermediate revision by the same user not shown)
Line 2: Line 2:
 
<partinfo>BBa_K200027 short</partinfo>
 
<partinfo>BBa_K200027 short</partinfo>
  
−
Testing construct to assay the production of the PAH enzyme
+
Testing construct to assay the production of the PAH enzyme. The PAH enzyme is responsible for the breakdown of phenylalanine into tyrosine. This enzyme is produced by the Human liver and a genetic deficiency of the encoding gene can lead to a condition called Phenylketonuria. This condition causes mental retardation through progessive brain damage caused by the accumulation of Phenylalanine.
 +
More detailed information on the enzyme at the main PAH registry page: [https://parts.igem.org/Part:BBa_K200008 BBa_K200008].
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
−
 
+
This composite part, comprising of subparts [https://parts.igem.org/Part:BBa_K200008 BBa_K200008], [https://parts.igem.org/Part:BBa_B0034 BBa_B0034], [https://parts.igem.org/Part:BBa_E0040 BBa_E0040], [https://parts.igem.org/Part:BBa_B0010 BBa_B0010] and [https://parts.igem.org/Part:BBa_B0012 BBa_B0012] was used in [http://2009.igem.org/Team:Imperial_College_London <i>The E.ncapsulator</i>] project by Imperial iGEM 2009.
 
<!-- -->
 
<!-- -->
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 17:26, 19 October 2009

PAH+RBS+GFP+TT

Testing construct to assay the production of the PAH enzyme. The PAH enzyme is responsible for the breakdown of phenylalanine into tyrosine. This enzyme is produced by the Human liver and a genetic deficiency of the encoding gene can lead to a condition called Phenylketonuria. This condition causes mental retardation through progessive brain damage caused by the accumulation of Phenylalanine. More detailed information on the enzyme at the main PAH registry page: BBa_K200008.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 287
    Illegal BamHI site found at 814
    Illegal XhoI site found at 524
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2032