Difference between revisions of "Part:BBa K203000"
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This part encodes the attachement of a GPI anchor, which allows targetting of proteins to which it is tagged to the Plasma Membrane. | This part encodes the attachement of a GPI anchor, which allows targetting of proteins to which it is tagged to the Plasma Membrane. | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
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<partinfo>BBa_K203000 SequenceAndFeatures</partinfo> | <partinfo>BBa_K203000 SequenceAndFeatures</partinfo> | ||
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===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K203000 parameters</partinfo> | <partinfo>BBa_K203000 parameters</partinfo> | ||
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| + | Shown to localize proteins to the plasma membrane. An expression device consisting of [[Part:BBa_I712004]] (CMV) [[Part:BBa_K203103]] (GFP), [[Part:BBa_K203000]] and [[Part:BBa_K203102]] (terminator) was assembled for this characterization. | ||
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| + | [[Image:GPI.jpg]] | ||
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Latest revision as of 09:25, 20 October 2009
Plasma membrane targeting (GPI anchor)
This part encodes the attachement of a GPI anchor, which allows targetting of proteins to which it is tagged to the Plasma Membrane.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 146
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 146
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 146
- 1000COMPATIBLE WITH RFC[1000]
Functional Parameters
| n/a | Plasma membrane targeting (GPI anchor) |
Shown to localize proteins to the plasma membrane. An expression device consisting of Part:BBa_I712004 (CMV) Part:BBa_K203103 (GFP), Part:BBa_K203000 and Part:BBa_K203102 (terminator) was assembled for this characterization.
