Difference between revisions of "Part:BBa K3717006:Experience"
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We used this part as a control of our experiment. We were able to express this part using a standard IPTG induction protocol. Cells were inducted with 0.1 mM IPTG and incubated for 18 hours overnight at 26 °C. Under our protocol, we were able to see significant overproduction of proteins. Most of which were in the pellet (unsoluble extract). We qualitatively tested the enzyme and detected an α-galactosidase activity. See https://2023.igem.wiki/ulaval/ for details. | We used this part as a control of our experiment. We were able to express this part using a standard IPTG induction protocol. Cells were inducted with 0.1 mM IPTG and incubated for 18 hours overnight at 26 °C. Under our protocol, we were able to see significant overproduction of proteins. Most of which were in the pellet (unsoluble extract). We qualitatively tested the enzyme and detected an α-galactosidase activity. See https://2023.igem.wiki/ulaval/ for details. | ||
| − | https://static.igem.wiki/teams/4726/wiki/results/ | + | https://static.igem.wiki/teams/4726/wiki/results/sds-page-purif-culot-lysat-ft-igem.png |
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| + | <b>Figure 1</b>. SDS-PAGE electrophoresis of lysis extracts. | ||
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| + | https://static.igem.wiki/teams/4726/wiki/results/sds-page-purif-wash-elution-igem.png | ||
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| + | <b>Figure 2</b>.SDS-PAGE electrophoresis of purification steps. | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 01:46, 10 October 2023
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K3717006
iGEM ULaval 2023
We used this part as a control of our experiment. We were able to express this part using a standard IPTG induction protocol. Cells were inducted with 0.1 mM IPTG and incubated for 18 hours overnight at 26 °C. Under our protocol, we were able to see significant overproduction of proteins. Most of which were in the pellet (unsoluble extract). We qualitatively tested the enzyme and detected an α-galactosidase activity. See https://2023.igem.wiki/ulaval/ for details.
Figure 1. SDS-PAGE electrophoresis of lysis extracts.
Figure 2.SDS-PAGE electrophoresis of purification steps.
User Reviews
UNIQ2176379216ef486d-partinfo-00000000-QINU UNIQ2176379216ef486d-partinfo-00000001-QINU